Genetic diversity of Taenia solium cysticerci from naturally infected pigs of central Mexico

Bobes, R., Fragoso, G., Reyes-Montes, María del Rocio, Duarte-Escalante, E., Vega, R., de Aluja, A., Zúñiga, G., Morales, J., Larralde, C. and Sciutto, E. (2010) Genetic diversity of Taenia solium cysticerci from naturally infected pigs of central Mexico. Veterinary Parasitology, 168 (1-2). pp. 130-135. ISSN 0304-4017.

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Abstract

This study was designed to explore if each individual case of naturally acquired porcine cysticercosis, living in different geographic rural areas of central Mexico, is caused by one or more different specimens of Taenia solium tapeworm. The genetic variability among cysticerci from the same pig and that from different pigs was assessed by random amplified polymorphic DNA markers (RAPDs), through the percentage of polymorphic loci, the number of effective alleles, the expected heterozygosity and the Shannon index. The parasite population’s reproductive structure was estimated through the association index (IA), and the degree of genetic differentiation and variation was determined using AMOVA. Using six different random primers, and a total of 181 cysticerci from 14 pigs, 88 different loci were amplified: 85% were polymorphic between pigs and 24% within pigs. The phenogram grouped the cysticerci into eight major clusters, with differences in the genetic distances among all cysticerci from 14 pigs ranging from 0.78 to 1. Most of the cysticerci grouped in accord with their different geographical origin and with their pig of origin. The similarity matrix produced from the phenogram (obtained by UPGMA) and the original similarity matrix yielded a good cophenetic correlation (r = 0.82317, P = 0.0004), which suggests that the phenogram accurately represents the original genetic similarities between isolates. The combination of IA (0.0–0.089) with the genetic diversity index (0.009–0.073) supports the idea that DNA diversity in T. solium cysticerci of naturally infected pigs is within the range expected from a recombination process occurring during sexual reproduction. The small genetic diversity found within the cysticerci of each pig (33.81%), when compared with that between pigs (66.19%), indicates that pigs are rarely infected by different tapeworms. It would then appear that porcine cysticercosis courses with effective concomitant immunity, as occurs in ovine cysticercosis.

Item Type: Article
Subjects: Q Science > Q Science (General)
Q Science > Q Science (General) > Q0002 General
Q Science > QH Natural history > QH0301 Biology (General) > QH0359 Evolution
Q Science > QL Zoology
Q Science > QL Zoology > QL0001 General
Q Science > QL Zoology > QL0360 Invertebrates
R Medicine > RB Pathology
R Medicine > RB Pathology > RB0127 Manifestations of disease
Divisions: Faculty of Social and Applied Sciences > School of Human and Life Sciences
Depositing User: Dr Rodrigo Vega
Date Deposited: 27 Apr 2017 14:00
Last Modified: 27 Apr 2017 14:07
URI: https://create.canterbury.ac.uk/id/eprint/15799

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Last edited: 29/06/2016 12:23:00