Cryopreservation of animal oocytes and embryos: current progress and future prospects

Mandawala, A., Harvey, S.C., Roy, T. and Fowler, K. (2016) Cryopreservation of animal oocytes and embryos: current progress and future prospects. Theriogenology, 86 (7). pp. 1637-1644. ISSN 0093-691X.

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Cryopreservation describes techniques that permit freezing and subsequent warming of biological samples without loss of viability. The application of cryopreservation in assisted reproductive technology encompasses the freezing of gametes, embryos and primordial germ cells. Whilst some protocols still rely on slow-freezing techniques, most now use vitrification, or ultra-rapid freezing, for both oocytes and embryos due to an associated decreased risk of damage caused by the lack of ice crystal formation, unlike in slow-freezing techniques. Vitrification has demonstrated its use in many applications, not only following in vitro fertilisation (IVF) procedures in human embryology clinics, but also following in vitro production (IVP) of embryos in agriculturally important, or endangered animal species, prior to embryo transfer. Here we review the various cryopreservation and vitrification technologies that are used in both humans and other animals and discuss the most recent innovations in vitrification with a particular emphasis on their applicability to animal embryology.

Item Type: Article
Subjects: Q Science > Q Science (General) > Q0002 General
Divisions: Faculty of Social and Applied Sciences > School of Human and Life Sciences
Depositing User: Katie Fowler
Date Deposited: 27 Jul 2016 13:39
Last Modified: 23 Aug 2018 08:45

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Last edited: 29/06/2016 12:23:00