Cell division is essential for elimination of the yeast [PSI+] prion by guanidine hydrochloride

Byrne, L., Cox, B., Coleman, D. A., Ridout, M., Morgan, B. and Tuiteq, M. (2007) Cell division is essential for elimination of the yeast [PSI+] prion by guanidine hydrochloride. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 104 (28). pp. 11688-11693. ISSN 1091-6490.

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Guanidine hydrochloride (Gdn·HCl) blocks the propagation of yeast prions by inhibiting Hsp104, a molecular chaperone that is absolutely required for yeast prion propagation. We had previously proposed that ongoing cell division is required for Gdn·HCl-induced loss of the [PSI+] prion. Subsequently, Wu et al.[Wu Y, Greene LE, Masison DC, Eisenberg E (2005) Proc Natl Acad Sci USA 102:12789–12794] claimed to show that Gdn·HCl can eliminate the [PSI+] prion from α-factor-arrested cells leading them to propose that in Gdn·HCl-treated cells the prion aggregates are degraded by an Hsp104-independent mechanism. Here we demonstrate that the results of Wu et al. can be explained by an unusually high rate of α-factor-induced cell death in the [PSI+] strain (780-1D) used in their studies. What appeared to be no growth in their experiments was actually no increase in total cell number in a dividing culture through a counterbalancing level of cell death. Using media-exchange experiments, we provide further support for our original proposal that elimination of the [PSI+] prion by Gdn·HCl requires ongoing cell division and that prions are not destroyed during or after the evident curing phase.

Item Type: Article
Subjects: Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry
Divisions: Faculty of Social and Applied Sciences > School of Human and Life Sciences
Depositing User: Dr Lee Byrne
Date Deposited: 14 May 2015 14:08
Last Modified: 14 May 2015 14:08
URI: https://create.canterbury.ac.uk/id/eprint/13340

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Last edited: 29/06/2016 12:23:00