Novel approach for deriving genome wide SNP analysis data from archived blood spots

Fowler, K., Reitter, C., Walling, G. and Griffin, D. (2012) Novel approach for deriving genome wide SNP analysis data from archived blood spots. BMC Research Notes, 5 (1). p. 503. ISSN 1756-0500.

Full text not available from this repository.

Abstract

BACKGROUND: The ability to transport and store DNA at room temperature in low volumes has the advantage of optimising cost, time and storage space. Blood spots on adapted filter papers are popular for this, with FTA (Flinders Technology Associates) Whatman™TM technology being one of the most recent. Plant material, plasmids, viral particles, bacteria and animal blood have been stored and transported successfully using this technology, however the method of porcine DNA extraction from FTA Whatman™TM cards is a relatively new approach, allowing nucleic acids to be ready for downstream applications such as PCR, whole genome amplification, sequencing and subsequent application to single nucleotide polymorphism microarrays has hitherto been under-explored. FINDINGS: DNA was extracted from FTA Whatman™TM cards (following adaptations of the manufacturer's instructions), whole genome amplified and subsequently analysed to validate the integrity of the DNA for downstream SNP analysis. DNA was successfully extracted from 288/288 samples and amplified by WGA. Allele dropout post WGA, was observed in less than 2% of samples and there was no clear evidence of amplification bias nor contamination. Acceptable call rates on porcine SNP chips were also achieved using DNA extracted and amplified in this way. CONCLUSIONS: DNA extracted from FTA Whatman cards is of a high enough quality and quantity following whole genomic amplification to perform meaningful SNP chip studies.

Item Type: Article
Divisions: pre Nov-2014 > Faculty of Social and Applied Sciences > Geographical and Life Sciences
Depositing User: Katie Fowler
Date Deposited: 25 Nov 2014 17:14
Last Modified: 11 Dec 2014 14:12
URI: https://create.canterbury.ac.uk/id/eprint/12931

Actions (login required)

Update Item (CReaTE staff only) Update Item (CReaTE staff only)

Downloads

Downloads per month over past year

View more statistics

Share

Connect with us

Last edited: 29/06/2016 12:23:00